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Background & Aims: During the previous decades increasing the hospital infections have taken into account, increasingly. In the United States, almost 1.7 million hospital cases and 99000 deaths is reported annually. Surfaces of medical instruments such as Blood pressure cuff, stethoscope and Dialysis machine can accelerate the infection transfer. In this research, determining the hygienic circumstances of instrument surfaces has been investigated in Qom Shahid Beheshti Hospital. Materials & Methods:This experimental study was performed during 10 weeks in two stages consisting of before and after intervention. The intervention was aimed to the cleaning program which is carried out routinely by hospital tenants. The assessment was applied two times a week with two methods including ICNA and ACC. The results were reported as “clean” and “dirty”. Statistical analysis was done with SPSS software version 18. Wilcoxon and Mc-Nemar tests were used. Results: Based on the ICNA method, 61% and 39.5% of all objects were “dirty” before and after the interventions, respectively. Whereas, based on the ACC method, 76% and 69.5% of all objects reported as “dirty” before and after the intervention, respectively . Results showed that, the intervention (cleaning program) had a significant impact on increasing the hygienic status according to both ICNA (Pvalue=0.00) and ACC (Pvalue

Keywords: Environmental health, Infection Control, ICU.

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