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Received: 1 Dec , 2015 Accepted: 4 Feb , 2016 Abstract Background & Aims: Previous studies showed that sucrose is a pain relief in infants, but a few studies was done about the effect of sucrose with different concentrations in IM injection, and their results are confusable. The aim of the current study was comparing the effect of oral sucrose 20%, 30% and 50% on relief of behavioral pain responses from Hepatitis B vaccine injection in full term infants. Material & Methods: In this randomized clinical trial, the subjects were divided into four groups randomly. 2 minutes before injection of Hepatitis B vaccine, in case groups 2 ml of one of the four sterile solutions namely sucrose 20%, 30% or 50% was given, and in the control group 2ml sterile distilled water was given with syringe into the baby's' mouth in less than one minute. Before, 2 and 7 minutes after giving sucrose, infants pain was assessed with NIPS. Results: The findings showed that pain severity in the 2 and 7 minutes groups, was significantly less than the control group (p

Keywords: pain, sucrose, infant

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